Pyramiding of <i>cry</i> toxins and methanol producing genes to increase insect resistance in cotton

The idea of enhanced methanol production from cell wall by pectin methyl esterase enzymes (PME) combined with expression cry genes Bacillus thuringiensis as a strategy to improve insect pest control in cotton is presented. We constructed cassette containing two (cry1Fa and Cry32Aa) pme genes, one Arabidopsis thaliana (AtPME), other Aspergillus. niger (AnPME) pCAMBIA1301 plant vector using CAMV-35S promoter. This construction was transformed Eagle-2 variety shoot apex-cut Agrobacterium-mediated transformation. Expression confirmed qPCR. Methanol measured the plants showing only plants, contrast non-transgenic plants. Finally, bioassays performed transgenic expressing showed 100% mortality for Helicoverpa armigera (cotton bollworm) larvae, 70% Pectinophora gossypiella (pink larvae 95% Earias fabia, (spotted that higher than 84%, 49% 79% mortality, respectively. These results demonstrate Bt. cry-genes coupled are an effective different pests.